Chemical Composition Identification and Comparison Antibacterial Activity of Some Essential Oils against Three Pathogenic Bacteria

Certainly, using herbal plants is the oldest way of mankind to treat the diseases. Considering the drug resistance and the side effects of chemical antibacterial drugs, the research approach is increasingly going toward using natural resources. Hence, this study aimed at examining the antimicrobial effect of Thymus vulgaris, Eucalyptus caesia Benth and Rosemarinous officinalis extracts against 3 species of pathogens and examining the effective components of the extracts.Antibacterial effect of T. vulgaris, E. caesia Benth and R. officinalis extracts was examined against 3 species of bacteria Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumonia using disk diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were evaluated at the times 24, 48 and 72 h. Also the effective components of the extracts were measured using Gas chromatography–mass spectrometry (GC-MS).Obtained results revealed that all 3 extracts possess inhibitory effect on used pathogenic species, but T. vulgaris extract show the highest effect compared with the other used extracts. Also comparison of the antibacterial effects of the extracts with 3 antibiotics Ampiciline, Penicillin and Tetracycline showed that the antibiotics possess a better effect in controlling the pathogens.Results of the present study showed us that T. vulgaris extract possess strong inhibitory effect on pathogenic bacteria, so it can be used as an antibiotic drug which derived from a herbal plant.